Interestingly, we observed that in addition to single immune checkpoint marker positive CD8+ T cell subsets (PD1+ T cells and Tim3+ T cells), CD8+ T cell subsets co-expressing two (Tim3+PD1+ T cells, CD38+Tim3+ T cells, and CD38+PD1+ T cells) or multiple immune checkpoint markers (PD1+CD38+Tim3+ T cells) were also abundant, albeit at different proportions, in bladder tumor tissues (Figure 1J). This evidence concerns the gene CD8A and urinary bladder neoplasm.