A heterozygous suspected pathogenic variant was identified in the individual with HSP described above, in which a GG pair of nucleotides in the open reading frame of KLC4 was deleted to cause a frame shift (NM_201523.2; c.1160-1161delGG; p.G369Afs*8) (Fig. S1). This evidence concerns the gene KLC4 and hereditary spastic paraplegia.