The results showed that Cell cycle was mainly enriched in HMGB1+ Granulosa; Cytokine-cytokine receptor interaction was mainly enriched in T cells, Monocytes and B cells; Chemokine signaling pathway was mainly enriched in T cells and Monocytes; Oocyte meiosis was mainly enriched in HMGB1+ Granulosa Tight junction was mainly enriched in KRT18+ Granulosa; Dilated cardiomyopathy was mainly enriched in muscle cells (Figure 5D). The gene discussed is HMGB1; the disease is dilated cardiomyopathy.