However, in the input NMFH2 lysates, the phosphorylated tyrosine levels in pCMV6-PAK1T423E and pCMV6-PAK1 transfectants were similar, implying the involvement of threonine activation and tyrosyl phosphorylation to facilitate PAK1 nuclear entry in myxofibrosarcomas. The gene discussed is PAK1; the disease is myxofibrosarcoma.