In our myxofibrosarcoma samples assembled in TMAs, PAK1 gene copy number and PAK1 mRNA abundance were assessed using FISH and bDISH assays, respectively, in which PAK1-amplified cases (Figure-1C), present in 15.5% (11/71) of cases informative for both parameters (Table-S1), exhibited a significantly higher PAK1 mRNA level (Figurge-1D, p<0.001), hence indicating PAK1 as a diver oncogene. This evidence concerns the gene PAK1 and myxofibrosarcoma.