ELISA demonstrated enhanced levels of the inflammatory factors IL-2, IL-15, TNF-α, and IFN-γ in the intratumoral TME in the BsAb-treated group compared with those in the other groups (Fig. S4D-G). In line with these results, enhanced infiltration of CD8-positive T cells and reduced percentages of MDSCs and M2 TAMs were found in the TME of the analyzed tumor specimens in the PD-1 scFv- and TREM2 scFv-treated groups. This evidence concerns the gene TNF and neoplasm.