Second, the signature gene of NII.clusterB-PNI,VCAM1,is need to explore in the future about the interaction with STAT3 in regulating the expression of PD-L1,which will be a hopeful biomarker to develop new treatments for GC patients.Third, we lacked a large prospective cohort to verify the validity and accuracy of our model's identification and prediction.However, we attempted to address this problem by validating our model with a large retrospective cohort and tissue samples. This evidence concerns the gene STAT3 and gastric cancer.