CD274 and melanoma: Importantly, we could confirm that RANO can induce this immunogenic phenotype as a single agent in the absence of vemurafenib, because it induced the expression of antigen presentation genes, interferons and PD-L1 in A375 cells and in BRAFV600E mouse melanoma cell lines 5555 and YUMM1.7 (Fig. 8a,b and Extended Data Fig. 7a,b).