Upon 6-h infection and 24 h-incubation in virus-free medium, VAP-A or ORP3-deficient cells produced no Gag-iGFP, as observed by immunocytochemistry (ICC) and CLSM, whereas Gag-iGFP was found in the cytoplasm of the scrambled shRNA and VAP-B-deficient cells (Fig. 3e). This evidence concerns the gene OSBPL3 and infection.