To better understand the relevance of the mechanism regulating HIV-1 IN entry into the NR and nucleoplasm, we investigated whether HIV-1 enters HeLa cells by endocytosis by pretreating them for 30 min with dynasore (DNS), a cell-permeable dynamin inhibitor that prevents the scission of clathrin-coated pits from plasma membrane48, before 3-h infection. The gene discussed is CD44; the disease is infection.