ACHE and aneurysm: In order to understand whether this pathway protects from aneurysm development, 6- to 8-week-old Yap1fl/fl × Wwtr1fl/fl mice with 1 allele of the Itga8-CreERT2 transgene (17) were injected intraperitoneally with tamoxifen for inducible KO of YAP and TAZ in vascular smooth muscle (i8-YT-KO) (Supplemental Figure 1, A and B).