IF assay showed that TLR4 and TNF-α cells expression increased in PD mice colon (P < 0.001, P < 0.001, vs. Normal) and significantly decreased after CDG therapy (P < 0.001, P < 0.01, vs. MPTP; Fig. 6A, B and D, E). This evidence concerns the gene TLR4 and Parkinson disease.