SLC12A2 and Dravet syndrome: In addition, due to the dependence of NKCC1 and KCC2’s function on their location at the cell membrane, subcellular-fractionation experiments were done to detect the heavy overexpression of NKCC1 in the synaptosomal membrane fraction of the tissue belonging to the Ts65Dn mice and human subjects with DS (p = 0.043 and 0.023, respectively [125].