However, we did previously report that at ~ 7–8 months of age (approximate age at which we noted sex differences in metabolic outcomes and hypothalamic inflammatory markers shown here), both male and female 3xTg-AD mice exhibited increased Iba1 labeling in the arcuate nucleus and dorsomedial hypothalamus, in addition to increased GFAP labeling in the arcuate nucleus and dorsomedial nucleus [34]. The gene discussed is AIF1; the disease is Alzheimer disease.