C, D FLS-RA were treated with nigericin by the concentration of 10 μM for 24h accompanied with siRNA-SMAD2 or not, the expression of ASC, NLRP3 were detected by Western blot. E, F Apoptosis rate of FLS-RA was detected by flow cytometry in three groups. The gene discussed is NLRP3; the disease is rheumatoid arthritis.