Turning our attention to pathway enrichment analysis for the full dataset, we first examined upregulated pathways which involved the above significant differentially expressed genes, BMPR2 and ESR1 (specifically KEGG pathways for estrogen signaling, fluid shear stress and atherosclerosis, hippo signaling, and TGF-beta signaling) (Supplementary Table 1). This evidence concerns the gene ESR1 and atherosclerosis.