In this retrospective study, we performed the evaluation of the Idylla GeneFusion system for the multiplex detection of ALK, ROS1, RET, NTRK1, NTRK2, NTRK3 fusions and MET exon 14 skipping in 35 clinical formalin-fixed paraffin-embedded (FFPE) specimens from NSCLC patients using IHC/FISH and RNASeq as the gold standards. Here, RET is linked to non-small cell lung carcinoma.