Colony formation (Fig. 2B) and cell growth curve assays (Supplementary Fig. 2A) demonstrated that knockdown of SRSF3 significantly decreased the proliferation ability of NPC cells, which was further confirmed by obviously reduced numbers of SRSF3-knockdown cells stained with EdU, a well-known proliferation marker (Fig. 2C). The gene discussed is SRSF3; the disease is nasopharyngeal carcinoma.