We first knocked down the expression of AMOTL1-L in NPC cell lines (S26 and 5–8 F) using two siRNAs specifically targeting the exon 12 of AMOTL1 (Fig. 5A and Supplementary Fig 3A, Supplementary File 1) and observed significantly inhibited proliferation ability of NPC cells with AMOTL1-L knockdown compared with the control group (Fig. 5B, Supplementary Fig. 3B), which was further confirmed by the reduced EdU (+) cells and decreased expression of CCND1 and c-MYC in the AMOTL1-L knockdown cells (Supplementary Fig. 3C, D, Supplementary File 1). This evidence concerns the gene CCND1 and nasopharyngeal carcinoma.