To systematically explore the roles of SRSFs in NPC, we first analyzed the mRNA expression levels of 12 classical SRSFs in 87 NPC tumors and 10 non-cancerous control samples using in-house transcriptome data, among which SRSF2, SRSF3, and SRSF9 were found to be upregulated in NPC samples compared with control samples (P < 0.01; Fig. 1A, B, and Supplementary Fig. 1A). This evidence concerns the gene SRSF3 and nasopharyngeal carcinoma.