In this respect, in our previous study by El-Sayed et al. (2023a), in vitro, we found that the myco-fabricated ZnO-NPs revealed excellent in vitro biological activities, including anti-inflammatory and acetylcholinesterase inhibitory potentials, so we need to apply these results in vivo. Thus, the aim of this investigation was to determine whether two distinct doses of ZnO-NPs had any positive effects on biochemical variables related to neurotransmission, oxidative stress, and inflammation in mice that had been given AlCl3 to cause AD. Here, ACHE is linked to Alzheimer disease.