Cytokines such as IL-6 and TGF-β, secreted by microglia, were cultured in the upper layer of the transwell system which directly stimulated the underlying neuroblastoma cells, and the synergistic PBM effect of the 808-nm and 1,064-nm laser irradiations showed the best therapeutic results (Figure 5), even reaching the level of the control group. Here, TGFB1 is linked to neuroblastoma.