To investigate whether TLR4 and PTK2 function in KLF7-mediated HCC metastasis, we downregulated TLR4 or PTK2 expression in KLF7-overexpressing PLC/PRF/5 cells and upregulated TLR4 or PTK2 in MHCC97H cells with KLF7 knockdown (Figure 2H). The gene discussed is PTK2; the disease is hepatocellular carcinoma.