Additionally, the orthotopic HCC models showed that the impact of knockdown or overexpression of TLR4 or PTK2 on HCC metastasis was less significant in the KLF7 non-manipulated state than in the KLF7 manipulated state, further demonstrating that the TLR4 and PTK2 effects are downstream of KLF7 (Figure S6). This evidence concerns the gene KLF7 and hepatocellular carcinoma.