HMGB1 and hepatocellular carcinoma: To determine whether proinflammatory factors triggered KLF7 overexpression in HCC cells, PLC/PRF/5 and Huh7 cells with low endogenous KLF7 expression were stimulated with a panel of inflammatory factors (IL-1β, IL-6, IL-17A, IL-8, TNF-α, TGF-β, LPS and HMGB1) enriched under chronic liver inflammation 19, 20.