In addition, a study using standardized L.E.M. (a mixture of polysaccharides, amino acids, lipids, and minerals) produced by culturing in liquid medium reported prolonged tumor development time, increased number of NK cells, and increased IFN-γ-producing tumor antigen-specific CTLs in the B16F0 melanoma inoculated mouse model [18]. This evidence concerns the gene IFNG and neoplasm.