Consistent with human cells, we manipulated murine CHAC1 by transduction of two different guide RNAs in multiple mouse tumor cell lines including Hepa1-6 (Supplementary Fig. 3d), B16F10 (Supplementary Fig. 3e), MC38 (Supplementary Fig. 3f ) and PanO2 (Supplementary Fig. 3g). This evidence concerns the gene CHAC1 and neoplasm.