Similarly, in various other tumor cell lines including OS-RC-2 (Supplementary Fig. 4f ), 786-O (Supplementary Fig. 4g) and SNU387 (Supplementary Fig. 4h) cells, forced CHAC1 expression was able to enhance ferroptosis induced by cystine deprivation or erastin treatment and recover ferroptosis inhibited by simultaneous methionine deprivation (Supplementary Figs. 4f–h). This evidence concerns the gene CHAC1 and neoplasm.