ACE and nephronophthisis: This technique has been used in diverse mice models such as healthy mice, nephrectomised mice, and animals with nephronophthisis [46]; C57BL/6, Balb/c, SV129, NMRI mice [43] lean and obese C57BL/6J mice [29] and animals lacking renal angiotensin-converting enzyme (ACE) [16].