As shown in Figure S6A, we observed that ZNRF2 was significantly negatively correlated with TMB in two tumours: COAD (p < 0.001), COADREAD (Colon adenocarcinoma/Rectum adenocarcinoma oesophageal carcinoma) (p < 0.001). This evidence concerns the gene ZNRF2 and rectum adenocarcinoma.