After treatment with 50 μg/mL ALAPP, the levels of expression of Cdk4 and Akt1 were unchanged in MC3T3 osteoblasts, but compared with levels in untreated cells, the levels of CDK4 and AKT1 expression were downregulated by about 42% and 43%, respectively, in MG-63 osteosarcoma cells (Figures 7C and 7D). The gene discussed is CDK4; the disease is osteosarcoma.