To evaluate the influence of SCXN on drug accumulation in tumors, a luciferase-expressing CT26 (CT26-luc) tumor-bearing mouse model was built and orally administrated with the infrared fluorescent dye 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindotricarbocyanine iodide (DiR)-loaded Sxy nanoparticle (Sxy-DiR) or as free DiR. Here, DDX53 is linked to neoplasm.