Similar to our observations in the dual-tumor ovarian cancer model above, we observed significant expansion and greater persistence of HER2-CAR/mbIL12 T cells in the peripheral blood of regional i.c.v. treated mice, as compared with HER2-CAR T cells alone (Fig. 4d, e), with persisting HER2-CAR/mbIL12 T cells in the blood showing a greater central memory phenotype (Fig. S19a, b), along with greater T-cell infiltration in both brain and bone metastases (Fig. 4f). The gene discussed is ERBB2; the disease is neoplasm.