We confirmed systemic toxicities associated with sIL12 injections, with mice showing signs of splenomegaly (Figs. 5e and S21, top), signs of liver abnormalities and greater T-cell infiltration in the liver (Figs. 5f and S21, bottom), along with increased serum ALT and AST levels and changes in peripheral blood lymphocyte counts (Fig. 5g, h), while no appreciable systemic effects were observed with TAG72-CAR/mbIL12 T cells. This evidence concerns the gene GPT and Splenomegaly.