When targeting the G. duodenalis tpi locus, 20 samples (26% of all samples with infectious status identified using tpi locus; 17% of positive infections using diagnostic 18S real-time PCR) were identified as single G. duodenalis assemblage A infections (Samama school, N = 8; Mchoka school, N = 7; Sungusya school, N = 3; and Malinde [St. Here, TPI1 is linked to infection.