Hits and analogs from these series disrupted AR interactions with p160 coactivators in mammalian 2-hybrid assays, inhibited transactivation driven by full length AR and/or AR-V7 splice variants, reduced endogenous and DHT-induced PSA biomarker expression and secretion by CRPC cells, and differentially inhibited the growth of AR positive PC cell lines. The gene discussed is AR; the disease is pachyonychia congenita.