TTN and myopathy: Missense variants in titin domains linked to myopathies have previously been shown to reduce the thermal stability of the domain by at least ∼ 17 °C (Rees et al., 2021), prevent soluble expression of the protein when expressed in E. coli (Hedberg et al., 2014), and/or resulted in a soluble but unfolded protein (Rudloff et al., 2015).