Moreover, inhibition of AMPK pathway also activated microglia and astrocytes, increased the production of TNF-α, NLRP3 and ASC, and reduced autophagy flux in the hippocampus from mice with high-fat feeding-triggered depression, while autophagy activator attenuated the above changes caused by high fat feeding [45]. This evidence concerns the gene NLRP3 and depressive symptom measurement.