C4B and Alzheimer disease: We also found significantly higher soluble pTau Thr 181 (Fig. 4c, left; t10 = 19.12, P < 0.0001), insoluble pTau Thr 181 (Fig. 4c, right; t10 = 18.76, P < 0.0001), soluble pTau S 396 (Fig. 4d, left; t10 = 13.58, P < 0.0001), and insoluble pTau S 396 (Fig. 4d, right; t10 = 7.053, P < 0.0001) in 3xTg-AD Ch− mice.