HCC markers like GPC3 and AFP, as well as hepatocyte markers like ALB, TTR, APOA1, and APOE, were found to be highly expressed in HCC organoids and corresponding tissues, whereas CC and ductal markers like EPCAM, KRT19, and S100A1186, were found to be overexpressed in CC organoids, which was compatible with the features of clinical primary liver cancers. This evidence concerns the gene GPC3 and cholangiocarcinoma.