Strikingly, sandwich ELISA using anti-CD9/CD63 mAbs and SN-131 (alkaline phosphatase-labelled) that PANC-1 cells release displaying cargo MUC1 TRD recognized by SN-131 (Fig. 4e–g and Fig. S5, ESI†), implicating that exosomes secreted from pancreatic cancer cells could interact directly with immune cells expressing Siglecs. This evidence concerns the gene CD63 and pancreatic neoplasm.