In this study,we present the production of a whole synthetic IgE molecule with selectivityfor amoxicillin, a commonly prescribed β-lactam antibiotic.By utilizing this synthetic IgE molecule as a standard, we aim toimprove the diagnostic accuracy of amoxicillin-specific IgE antibodies,representing a significant advancement in reliable and standardizedimmunoassays for β-lactam antibiotic allergy testing. Here, IGHE is linked to allergic disease.