The dysregulated liver metabolism, lipid deposition, hepatosteatosis, and profibrosis, followed by elevated pro‐inflammatory cytokines and abnormal liver functional indicators were further observed by Zdhhc3‐HepTG over the course of HFHC‐induced NASH phenotype (Figure S10k,l, Supporting Information). Here, ZDHHC3 is linked to metabolic dysfunction-associated steatohepatitis.