Inversely, in line with the similar protocols mentioned in Figure S8, Supporting Information, overexpression of ZDHHC3 induced by adenovirus in THLE2 cells and mouse primary hepatocytes, a significant increase in lipid deposition, and inflammation in vitro upon challenge with PAOA or NASH serum could be visualized by ectopically overexpressed ZDHHC3 cells (Figure S11a–h, Supporting Information). This evidence concerns the gene ZDHHC3 and metabolic dysfunction-associated steatohepatitis.