Combined with the results of PPI network analysis, PTGS2, EGFR, AKT1, ESR1, CASP3, MMP9, IL6, VEGFA, TNF, TP53, and HSP90AA1 may be the key targets of THSWD in the treatment of varicocele-associated male infertility. The gene discussed is TP53; the disease is male infertility.