To this aim, we carried out a colocalization analysis of Mtb DsRed with: (i) ubiquitin (Fig. 3A, D); (ii) the ubiquitin-binding autophagy adapter NDP52 (Fig. 3B, E), which is also recruited to Mtb upon K63-linked polyubiquitination mediated by Parkin [24]; and (iii) the autophagosome marker LC3 (Fig. 3C, F) in GFP and TRIM32 expressing THP-1 24 h post infection by confocal microscopy. Here, PRKN is linked to infection.