To explore the possibility that SerpinB2, vWF, and C3 are potential biomarkers of therapy-induced senescence in human leukemia, 3 human AML cell lines, OCI-AML4, OCI-AML5, and OCIM2 were treated with Dox for 24 h and high levels of SA-β-gal positive cells were detected 3 days after drug treatment (Supplementary Fig. 7B). This evidence concerns the gene C3 and acute myeloid leukemia.