To understand cellular programs driven by BRAF(V600E) for HCL pathogenesis, we extracted mRNAs from splenic leukemic cells in HSCVE, BVEP53−/− and BVEPTEN−/− mice and determined gene expression profiles by RNA-seq. As shown in Fig. 5A and B, splenic leukemic cells in these mice strains had quite different gene expression patterns though having significant co-regulated genes. Here, BRAF is linked to hairy cell leukemia.