To explore the glycosylation status of PTPRZ in glioma, we treated sPTPRZ in CSF from glioma patients with several kinds of glycosidases: chondroitinase ABC (ChABC), end-β-galactosidase, sialidase, and peptide-N-glycosidase (PNGase). This evidence concerns the gene NGLY1 and glioma.