Kaplan–Meier survival curve OS analysis was performed on 315 HCC patient samples, the results showed that high expression of STMN1 (p < 0.0001), CLSPN (p = 0.00096), MDK (p = 0.0032), RNFT2 (p = 0.0023), PRR11 (p = 0.00064), and RNF157 (p = 0.0056) was associated with worse prognosis, low expression of GHR (p = 0.00093) was associated with worse prognosis (Fig. 8B). The gene discussed is CLSPN; the disease is hepatocellular carcinoma.