Pai1-Tg mice demonstrated down-regulated secretion of ACH in the serum (Fig. 6B) and down-regulated mRNA and protein levels of AQP5 (Fig. 6C and E, and 6 F) and CACNA1C (Fig. 6D and E, and 6G) in sweat glands of hydrochloride-induced hyperhidrosis when compared with normal mice exposure to hydrochloride. Here, CACNA1C is linked to Hyperhidrosis.