PAI-039 could up-regulate the relative expression of CHRNA1 in both mRNA (Fig. 2A) and protein levels (Fig. 2B C) in hydrochloride-induced hyperhidrosis mice, while PAI-039 did not alter the relevant CHRNA1 expression in normal mice without hydrochloride stimulation. This evidence concerns the gene CHRNA1 and Hyperhidrosis.