Briefly, we genotyped F2 generation of WT and xcp1 crossed plant (WT × xcp1) lines and cultivated specific F2 lines F2–5, F2–3, F2–8, and F2–13 for F3 homozygous WT XCP1 (F2-5-F3 XCP1w/w) and mutated XCP1 (F2–3–F3 XCP1m/m and F2–8–F3 XCP1m/m), and heterozygous mutated XCP1 (F2–13–F3 XCP1w/m) plants for CNYDase and pathogen infection assays (Supplementary Fig. 13). Here, RETN is linked to infection.