Trichrome staining indicated no differences in fibrosis in the CRC lesions of Apc−/− and Apc−/−;Pkd1−/− CRC mice, although a trend to higher deposition in the submucosal layers of the colons of Apc−/−/ Pkd1−/− mice (Fig. S2A). This evidence concerns the gene PKD1 and colorectal carcinoma.