Experimentally, we reveal that TMPO-AS1L acts as scaffold to strengthen the interaction between casein kinase 2 alpha 1 (CSNK2A1) and DEAD-box helicase 3 X-linked (DDX3X), and activates the Wnt/β-catenin signaling pathway, which reciprocally promotes PCa BM. Here, CSNK2A1 is linked to posterior cortical atrophy.