We then constructed Tollip KO CRC cell lines using the CRISPR‐Cas9 system and found that the degradation of MCT1 was significantly reduced by CHX treatment in Tollip KO cell lines (Figure S4d, Supporting Information and Figure 4d,e), suggesting that cargo receptor Tollip mediates the selective autophagic degradation of MCT1. The gene discussed is TOLLIP; the disease is colorectal carcinoma.