In addition, an increase in CD47 expression in U87 human GBM cells was also observed by ectopically expressing an activated EGFRvIII mutant, which is frequently detected in human GBM (Figure 2B).[8] Pretreatment of U251 human GBM (Figure 2C) and CT‐2A mouse glioma (Figure S2C, Supporting Information) cells with the EGFR inhibitors afatinib, AZD9291, or AZD3759 blocked EGF‐induced CD47 expression. This evidence concerns the gene EGF and central nervous system cancer.