DARS1 and glioblastoma: We found that siRNA/shRNA-mediated suppression of DARS1-AS1 expression led to a reduction in the number of colonies formed by GBM cells in clonogenic assay as well as a marked decrease in the number of colonies formed by GSCs in soft agar colony formation assay (Materials and Methods), following x-ray radiation treatment (Fig. 8, I to L, and fig.