Further study showed that hirudin caused a decrease in the phosphorylation of mTOR and its downstream factors ULK1, P70S6K and 4EBP1 and preliminarily showed an inhibitory effect of hirudin on glioma via cell‐derived xenografts (CDXs) in nude mice in vivo. The gene discussed is EIF4EBP1; the disease is central nervous system cancer.