Here, we utilized our previously published ISDoT (In Situ Decellularization of Tissues) method (45) in order to isolate and enrich native ECM proteins from pancreatic and hepatic tissue during pancreatic cancer progression including pancreatic intraepithelial neoplasia (PanIN) and PDAC stages in the KPC (Tg (Pdx1-cre) 6TuvKrastm4TyjTrp53tm2Tyj) mouse model. This evidence concerns the gene PDX1 and familial pancreatic carcinoma.